Artikel Jurnal

Effects of probiotics and high fructose diet on De Ritis ratio, TGF-B levels, and liver histopathology of Sprague Dawley rats

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Background: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. Histopathological diagnosis
has been the gold standard for diagnosing and determining the severity of non-alcoholic steatohepatitis (NASH). However,
this method is invasive, especially for diagnosing NASH in children. This research aimed to study the effect of probiotics on
the De Ritis ratio, TGF-β levels, and histopathological changes in rats given High Fat, High Fructose (HFHFr) diet to look at the
correlation between the De Ritis ratio and liver histopathology in diagnosing NAFLD.
Method: Twenty-one male Sprague Dawley rats were included and divided into three groups. In the first group, as control
only received a normal chow diet for eight weeks. The second group received an HFHFr diet for eight weeks, and the last
group received HFHFr for eight weeks and a combination of HFHFr and probiotics for another eight weeks. After decapitation,
we took 3 ml of serum from each rat to measure AST, ALT, and TGF-β. A 4 μm thick liver tissue slide was taken and stained
with Hematoxylin – Eosin Stain for histopathological analysis. We used the Non-Alcoholic Steatohepatitis (NAS) Score for
measuring liver damage progression.
Results: De Ritis ratio and TGF-β level did not significantly differ between probiotic and non-probiotic groups (p = 0.064 and
0.383), but there was a significant NAS score difference (p = 0.001) in probiotic and non-probiotic groups. This was followed
by a significant correlation between the De Ritis ratio and the NAS score (r = 0.613, p = 0.003).
Conclusion:Probiotic supplementation alleviated liver damage caused by the HFHFr diet but did not successfully reduce the
De Ritis ratio or improve TGF-β

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Availability

Detail Information

Series Title

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Call Number

616.36 NIN e

Publisher

Bali : Bali Medical Journal., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

616.36

Content Type

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Media Type

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Carrier Type

-

Edition

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Subject(s)

Probiotics
Fructose
Liver Diseases

Specific Detail Info

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Statement of Responsibility

Ninung Rose Diana Kusumawati

Viewer

50

Other Information

Tanggal Pelaksanaan - Dari

2024-12-27

Tanggal Pelaksanaan - Sampai

2024-12-27

Akreditasi

Tidak

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